Fig 6.

Electrophysiological features of I_to, I_K1 and I_f currents in the neonatal cardiomyocytes cultured for 24 and 48 hrs in the absence (control) or presence of RLX. I-V plots of: (A) outward I_to, and (B) transient inward rectifier I_K1, evaluated by a voltage ramp protocol. (C) I_f trace currents in cardiomyocytes cultured for 24 hrs in the absence (a) or presence of RLX (b) and the relevant I–V plots at 24 and 48 hrs (c). RLX increases I_K1 size by shifting its voltage dependence towards more positive potentials and enhances the size of I_to and I_f. The related Boltzmann parameters and the statistical analysis are reported in Table 2. Data are mean values from 26 to 35 different cells.