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. 2012 Feb 28;16(3):569–581. doi: 10.1111/j.1582-4934.2011.01343.x

Fig 6.

Fig 6

Cell killing activity of NK-92-scFv(ch14.18)-ζ cells towards GD2-expressing melanoma and breast carcinoma cells. (A) Surface expression of GD2 on SK-Mel-23 and NW1539 melanoma cells and SK-BR-3 breast carcinoma cells was determined by flow cytometry using PE-Cy5-labelled GD2-specific mAb 14.G2a (filled areas). Control cells were treated with isotype control (open areas). (B) Cytotoxic activity of NK-92-scFv(ch14.18)LH-ζ towards SK-Mel-23 and SK-BR-3 cells was determined in europium release assays at an E/T ratio of 10:1. Parental NK-92 cells were included for comparison. Data are represented as mean ± S.D. (C) Cytotoxic activity of NK-92-scFv(ch14.18)HL-ζ and NK-92-scFv(ch14.18)LH-ζ towards NW1539 melanoma cells was investigated by microscopical analysis after 18 hrs of co-culture at low E/T ratios of 1:1 and 0.3:1. Parental NK-92 cells were included for comparison. Control cells were incubated in the absence of NK cells, or were treated with 8 μM of the apoptosis-inducing drug staurosporine as indicated. Representative fields are shown.