Fig 2.

Menin represses proliferation and migration of melanoma cells partly through PTN signalling. (A) Men1, PTN, RPTP β/ζ, VEGF, VEGFc and bFGF mRNA levels were detected by RT-PCR. (B) The efficiency of menin overexpression and the effect of Men1 expression on PTN, RPTP β/ζ and VEGF expression were determined by Western blotting and β-actin was used as loading control. (C) B16 cells were transfected with either vector expressing shRNAs against Luc or one of the two shRNAs against PTN and selected by G418. The efficiency of PTN silencing was determined by RT-PCR. (D) The proliferation of the selected B16 cells was estimated by MTT assay. (E) The selected B16 cells were added to upper filter and cell migration was determined. (F and G) B16 cells were transfected with either vector expressing shRNAs against Luc or one of the there shRNAs against RPTP β/ζ and selected by G418. The efficiency of RPTP β/ζ silencing was determined by RT-PCR and Western blotting. (H) The selected B16 cells were added to upper filter and cell migration was determined.