Fig 6.

Methylation of the menin promoter correlates with menin expression in A375 cell. (A) Primers for unmethylated and methylated DNA of corresponding CpG islands were used. (B) qMSP assay of MEN1 gene in A375 cells. (C) A375 Cells were treated with 5′-aza-dc at 3 or 5 μM for 7 days with medium changed each day, and MEN1 mRNA level was determined by real-time qPCR. (D and E) ChIP assay to demonstrate the association of DNMT with the MEN1 genes. (F) A375 cells treated with 5′-aza-dc at 5 μM for 7 days were added to the upper filter, and cell migration was determined. (G and H) The proliferation of A375 cells treated with 5′-aza-dc at 5 μM for 7 days was estimated by MTT assay and BrdU cell proliferation assay, respectively.