Fig 4.

Potentiation of chrysin-induced caspase-7 cleavage by GRP78 knockdown. (A) HepG2 cells were transfected with negative control siRNA (siCtrl) or siRNA to GRP78 (siGRP78). Forty-eight hours later, the cells were treated with 10 μM chrysin for further 24 hrs. Total proteins were harvested and subjected to Western blot analysis of cleaved caspase-7, GRP78 and β-actin. (B) SMMC-7721 cells were transfected with negative control siRNA (siCtrl) or siRNA to GRP78 (siGRP78). Forty-eight hours later, the cells were treated with 20 μM chrysin for further 24 hrs. Total proteins were harvested and subjected to Western blot analysis of cleaved caspase-7, GRP78 and β-actin.