Fig 5.

Assessment of the mechanism of internalization of EC-Fc/BNC in SK-BR-3 cells. (A) SK-BR-3 cells and SK-OV-3 cells were stained with antibodies against ErbB2 (green) and Cav-1 (red). (B) SK-BR-3 cells were treated with EC-Fc/BNC in the presence or absence of 100 nM of CPZ and stained with anti-human IgG antibody labelled with FITC. Transferrin-RITC was used as a control for the internalization. (C) SK-BR-3 cells were treated with EC-Fc/BNC labelled with RITC in the presence or absence of mβCD. The cells were stained with anti–EEA-1 antibody followed by secondary antibody against mouse IgG labelled with AlexaFlour-488. The cells were then stripped with and without acid treatment to remove the surface bound fraction and to visualize the internalized fraction. Bars, 10 μm.