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Use of HDACi leads to re-expression of ZAP70. (A) Namalwa cell line was treated with 1 or 10 mM valproic acid or vehicle control for 48 hrs; total RNA was prepared from both samples and used for RT-PCR detection of ZAP70 expression. (B) Namalwa cell line was treated with 1 or 10 μM MS-275 or vehicle control for 48 hrs; total RNA was prepared from both samples and expression of ZAP70 mRNAs was quantified and normalized to GAPDH. Results are averages ± S.E. (n = 3).
