Fig 4.
Allogeneic mesenchymal stem cells (MSCs) elicit an antibody response. LEW rats were injected with 1 × 106 syngeneic or allogeneic (DA) MSCs or allogeneic T cells. Serum was harvested 2–3 weeks after injection. Splenocytes of allogeneic DA rats were incubated with serum aliquots and, consequently, bound alloantigen-specific antibodies were stained with anti-rat IgM (A), anti-rat IgG1 (B) or anti-rat IgG2 (C) antibodies. B cells were excluded from analysis of IgM by counterstaining with CD45RA (n = 6 animals/group; *P < 0.05; **P < 0.01; n.s.: not significant; non-parametric Mann–Whitney test).