Fig 5.
Allogeneic mesenchymal stem cell (MSC)-induced antibodies mediate significant albeit reduced complement activation. LEW rats were injected with 1 × 106 syngeneic or allogeneic MSCs or allogeneic T cells. Serum was harvested 2–3 weeks after injection. Splenocytes of allogeneic DA rats were incubated with serum (diluted 1:10) and treated with baby rabbit complement (diluted 1:10). Splenocytes were stained with PI to detect complement-mediated lysis. The grey bars show the means and 95% confidence intervals of serums from six animals per group tested and the black dots show means of triplicates from serum of individual animals. *P < 0.05, **P < 0.01 compared to LEW MSC and naïve; ##P < 0.01 compared to DATc (non-parametric Mann–Whitney test).