Fig 5.

Effects of cystamine treatment on expression level of pro-apoptotic proteins and survival signal. LV samples were obtained from NZB/W-F1 mice and Balb/c mice, which were treated with PBS or cystamine as described in Materials and methods. (A) Immunoblot for expression level of Bcl-2, Bad, phosphorylated Bad (p-Bad) and truncated Bid (tBid). (B) Quantitative data for tested targets from NZB/W-F1 mice were obtained by densitometric analysis and presented as ratio of the tested protein/α-tubulin (α-TN). (C) Immunoblot for level of phosphorylation of Erk1/2 (p-Erk1/2), quantitative data were obtained by densitometric analysis and presented as ratio of p-Erk1/2/α-TN. *P < 0.05 as compared to PBS control. n.s.: not significant.