Fig 6.

Effects of cystamine treatment on mitochondrial apoptotic signals. LV samples were obtained from NZB/W-F1 mice and Balb/c mice, which were treated with PBS or cystamine as described in Materials and methods. (A) Immunoblot for level of cytochrome c (Cyt-c), Apaf-1, active caspase-9 and active caspase-3 using specific antibodies. (B) Quantitative data for tested targets from NZB/W-F1 mice were obtained by densitometric analysis and presented as a ratio of tested target/α-tubulin (α-TN). *P < 0.05 and **P < 0.005 as compared to PBS controls.