Fig 1.

Effects of Raf-1 knockdown on hESC cell morphology. (A) Immunofluorescence analysis of cytoskeleton by vimentin (green), phalloidin (red) and merge (green and red), 48 hrs after transfection of Co-hESC, Eu-hESC and Ec-hESC cells with siRNA are shown, scale bar line = 100 μm. (B) Average number of cells with contracted phenotype (estimated by two independent investigators) out of three biological replicates are given as% of cells with contracted phenotype relative to the total cell number (set to 100%), **P < 0.005.