Fig 2.

Raf-1 regulates hESC morphology and migration. (A) Immunofluorescence analysis of cytoskeleton by phalloidin (red), Raf-1 cellular levels (green) and merge (green and red) 48 and 144 hrs after transfection of Eu-hESC cells with siRNA are shown on the left. Average number of cells with contracted phenotype (estimated by two independent investigators) out of three biological triplicates are given on the right as % of cells with contracted phenotype relative to the total cell number (set to 100%, ± S.D.). **P < 0.005; scale bar line = 100 μm. (B) Effects of Raf-1 knockdown on cell migration in Eu-hESC 48 and 144 hrs after initial transfection with either control (transfection reagent only) or control siRNA or Raf-1 siRNA are given. Average values of biological triplicates are shown as percentage of migrating cells relative to the controls (mean value set to 100%), **P < 0.005. (C) Raf-1 knockdown does not change the migration rate of Ec-hESC. A migration assay, 48 hrs after Raf-1 knockdown in Ec-hESC, was performed. Average values of biological triplicates are shown as percentage of migrating cells relative to the siRNA controls (mean value set to 100%).