Fig 5.

Effects of K5-N,OSepi on neutrophil infiltration and cytokines production induced by regional I/R. Myeloperoxidase (MPO) activity (A), ICAM-1 expression (B), PGE₂ (C) and TNF-α (D) levels were measured in myocardial homogenates of sham-operated rats (Sham) and rats that underwent 30 min. ischaemia followed by 1 or 24 hr reperfusion (vehicle). K5-N,OSepi (0.1–1 mg/kg in panels (A, C and D) and 1 mg/kg in panel (B) or B4/100 (1 mg/kg) were administered 15 min. before reperfusion. Densitometric analysis of the bands is expressed as relative optical density (O.D.) corrected for the corresponding β-actin contents and normalized using the related sham-operated band. Data are means ± S.E.M. of eight animals/group for MPO, PGE₂ and TNF-α and three separate experiments for Western blot analysis. *P < 0.01 versus vehicle (1h reperfusion) and •P < 0.01 versus vehicle (24 hrs reperfusion).