Fig 7.

Effects of K5-N,OSepi on markers of apoptosis in the hearts of rats that underwent myocardial I/R injury. Caspase 3 activity (A) and expression of the apoptotic markers Bid (B), Bax (C) and Bcl-2 (D) were measured in the hearts from sham-operated rats (Sham), rats subjected to 30-min. myocardial ischaemia followed by 1-hr reperfusion (vehicle) or rats subjected to myocardial I/R and treated with K5-N,OSepi (1 mg/kg) or B4/100 (1 mg/kg) treatment. Each immunoblot is from a single experiment and is representative of three separate experiments. Densitometric analysis of the bands is expressed as relative optical density (O.D.) corrected for the corresponding β-actin contents and normalized using the related sham-operated band. Data are means ± S.E.M. of four animals/group for caspase-3 activity and three separate experiments for Western blot analysis. *P < 0.05 versus vehicle.