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Scleroderma fibroblasts with constitutive Smad1 phosphorylation have high levels of cav-1 Primary cultures of human dermal fibroblasts from matched pairs of normal (N1-5) and diffuse scleroderma patients (S1-5) were grown to subconfluence and lysates were analysed by Western blot for caveolin-1 (cav-1), Smad1, phospho-Smad1, and β-actin.
