Fig 4.

Cav-1 overexpression increases CCN2 protein levels, while cav-1 depletion decreases both endogenous and TGFβ induced CCN2 protein in normal dermal fibroblasts. (A) Human dermal fibroblasts were grown to confluence and transduced with either cav1-Ad or control adenovirus (LucAd) for 24 hrs, followed by serum starvation for 24 hrs. A dose of cav1-Ad corresponding to a 2- to 4-fold increase in cav-1 protein was used, and a representative Western blot shows levels of cav-1, Smad1, phospho-Smad1, CCN2 and β-actin (left panel). (B) Human dermal fibroblast cultures were treated with scrambled siRNA (scr) or siRNA targeted against cav-1, serum starved and treated with or without TGFβ for 48 hrs prior to collection of cell lysates. Protein levels of cav-1, CCN2, and β-actin in the lysate were determined by Western blot.