Fig 3.

Scriptaid induces glioma cell-cycle arrest. (A) Scriptaid increases the expression of p21, p27 and decreased cMyc level in glioma cells in a dosedependent manner. LN229 and T98G cells were treated with different concentration of scriptaid for 24 hrs and Western blot analysis was performed to detect the expression of cell-cycle regulatory proteins p21, p27 and cMyc. A representative blot from three independent experiments with identical results is shown. Blots were reprobed for β-actin to establish equivalent loading. (B) Treatment of glioma cells with scriptaid induces G2 arrest in the cell cycle. FACS analysis was performed on cells treated with scriptaid and the percentages of cells in the various cell-cycle phases were plotted. FACS analysis profiles of scriptaidtreated cells are shown. Insets indicate percentage cells in G1, S and G2/M phases of the cell cycle in T98G cells. C and S denote control and scriptaid, respectively.