Fig 4.

Scriptaid induces DNA damage in glioma cells. (A) Scriptaid affects the ability of glioma cells to repair DNA damage. Ability of scriptaid-treated glioma cells to participate in DNA damage response was detected by determining the expression of γ-H2AX (Ser¹³⁹) and H2AX by Western blot analysis. A representative blot from three independent experiments with identical results is shown. Blots were reprobed for β-actin to establish equivalent loading. (B) Treatment of glioma cells with scriptaid increases DNA damage as determined by comet assay. The figure is a representative of comet assay performed on control and scriptaid-treated T98G and LN229 cells.