Fig 6.

The pro-apoptotic effect of scriptaid is mediated by JNK. (A) Scriptaid increases p38MAPK and JNK phosphorylation in glioma cells. Western blot analysis was performed to detect the expression of p38MAPK, phosphop38MAPK, JNK and pJNK in glioma cells treated with increasing concentration of scriptaid. Representative blot is shown from three independent experiments with identical results. Blots were reprobed for β-actin to establish equivalent loading. (B) Scriptaid-induced cell death is significantly attenuated in the presence of JNK inhibitor. The graph represents the percentage of viable cells as determined by MTS assay, observed when glioma cells were treated with scriptaid in the presence or absence of JNK inhibitor. Cells were treated with 20 μM scriptaid alone or in combination with 20 μM SP600125 for 24 hrs. Values represent the means ± SEM from three independent experiments. * Significant change from control; #significant change from scriptaid-treated cells (P≤ 0.05).