Fig 7.

Scriptaid-mediated DNA damage response and alteration in cellcycle regulatory molecules in glioma cells are JNK dependent. Glioma cells were treated with 20 μM scriptaid in the presence or absence of 20 μM JNK inhibitor SP600125, and Western blot analysis was performed. A decrease in pJNK, p21, p27 and H2AX expression respectively was observed in cells treated with scriptaid in the presence of JNK inhibitor, as compared to those treated with Scripatid alone. Representative blot is shown from three independent experiments with identical results. Blots were reprobed for β-actin to establish equivalent loading.