Fig 5.

NK-92 cytotoxicity inhibition induced by HLA-G expression. (A) Comparison of the mean cytolytic percentage of NK-92 to different target cells (Hep-G2 and Hep-G2-G). (B) Restoration of cytotoxicity by anti-HLA-G mAb 87G and anti-HLA class I mAb W6/32 blockade. (C) Restoration of NK-92 cytotoxicity by anti-ILT2 mAb GHI/75 blocking. Target cell Hep-G2 and Hep-G2-G cells were pre-incubated with 10 μg/ml mAb 87G, W6/32 and GHI/75, respectively. Isotopes IgG1 (for mAb 87G and mAb W6/32) and IgG2b (for mAb GHI/75) were used as internal controls. Experiments were performed in quadruplicate with an effector/target ratio of 20:1, and the results were expressed as percentage of specific lysis ± S.D.