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. 2008 Mar 19;13(1):164–176. doi: 10.1111/j.1582-4934.2008.00310.x

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Detection of TNFα receptor 2 (TNF-R2) in colonic mucosal tissue. (A) Immunoreactivity for TNF-R2 was absent in the colon of non-transgenic F344. (B, C) Strong immunopositivity in the colon of IgG2a, k-treated B27TR, both at 18 and 27 weeks of age. Macrophages and mast cells (arrows) and intestinal epithelial cells (arrowhead) were strongly positive. (D) The treatment with anti-TNFα mAb prevented the increased positivity for TNF-R2. Brown colour: DAB immunohistochemical developing; Red colour: AEC immunohistochemical developing; Blue colour: haematoxylin counterstain. Original magnification A, B ×40; C, D ×20. (E, F) RT-PCR results for TNF-R2. Low TNF-R2 mRNA expression was detected in F344 colon specimens, while up-regulation was observed in IgG2a, k-treated B27TR, both at 18 and 27 weeks. Both early and late anti-TNFα-treated rats showed low levels of TNF-R2 mRNA. Data are representative of at least three separate experiments. *P < 0.05 IgG2a, k-treated B27TR versus F344 and anti-TNFα-treated B27TR.