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TUNEL assay. (A) TUNEL-positive cells were found in the surface epithelium and crypts (arrows) of the colon in IgG2a,k-treated HLA-B27 transgenic rats. (B) In anti-TNFα-treated HLA-B27 transgenic rats, apoptotic cells are found mainly in the lamina propria (arrow) of the colon. Brown colour: DAB developing; Green colour: Fast Green counterstain. Original magnification × 20.
