2.

Primers used for semiquantitative RT-PCR

Gene name	Primer sequences	Product size	GenBank accession number
TNF-R1	Forward 5′-ACCAAGTGCCACAAAGGAAC-3′	249 bp	NM_013091
Reverse 5′-CTGGAAATGCGTCTCACTCA-3′
TNF-R2	Forward 5′-AAATGCAAGCACAGATGCAG-3′	244 bp	NM_130426
Reverse 5′-CAGCAGACCCAGAGTTGTCA-3′
GAPDH	Forward 5′-AGACAGCCGCATCTTCTTGT-3′	300 bp	NM_017008
Reverse 5′-CTTGCCGTGGGTAGAGTCAT-3′