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. Author manuscript; available in PMC: 2014 Oct 4.
Published in final edited form as: J Proteome Res. 2013 Sep 6;12(10):10.1021/pr400201d. doi: 10.1021/pr400201d

Figure 3.

Figure 3

MS/MS spectra and extracted ion chromatogram (XIC) of the unmodified and oxidized ATM Cys2074 peptides. A, The XIC plot vs. retention time of unmodified and modified cysteine variants of ATM Cys 2074 peptide QAGIIQALQNLGLCHILSVYLK demonstrates chromatographic separation, as follows: unmodified (m/z 799.1,+3) sulfinic acid (m/z 809.78,+3) and sulfonic acid (m/z 1222.17,+2) B, The MS/MS spectra of the QAGIIQALQNLGLCHILSVYLK precursor ions for sulfinic acid (m/z 809.78,+3) and sulfonic acid (m/z 1222.17,+2) modified peptides. The sulfinic acid containing peptide fragmented to produce the characteristic 66 Da neutral loss of HS(O)OH.