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(a) Comparison of ultraviolet radiation (UVR)-induced aggregation of W:F mutant constructs of HγD-Crys by monitoring OD600 of 0.5 mg mL−1 protein solutions in sample buffer over irradiation time. (b) Comparison of the concentration dependences of UVR-induced aggregation of W:F mutant HγD-Crys constructs by analyzing the apparent rate of aggregation (steepest linear slope of OD600 curves) versus protein concentration.
