Fig 1.

Differentiation of rabbit MSCs into specific cell lineages. (A) MSCs (a–e), osteoblasts (f–j), chondroblasts (k–o) and adipocytes (p–t) were left unstained (a, f, k and p), or stained with Alizarin Red (day 21, b, g, l and q), NBT-BCIP (day 14, c, h, m and r), Alcian blue (day 27, d, i, n and s) or Oil Red O (day 27, e, j, o and t). Scale bar ∇ 50 μm. (B) Quantitative RT-PCR analysis of mRNA expression of the indicated lineage-associated markers. MSCs, osteoblasts, chondroblasts and adipocytes specifically expressed Vimentin, Runx2, SOX9 and PPAR-gamma3, respectively. RQ: relative mRNA expression normalized to GAPDH.