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Cross talk between CAIX and CAXII expression. Reduced expression of CAIX by doxycycline (DOX)-induced shRNA interference in LS174Tr human colon carcinoma cells (LS-shca9) leads to up-regulation of CAXII. Co-staining for CAIX (Immunofluorescence) (upper panel) and CAXII (Immunohistochemistry) (lower panel) was observed in mice xenograft tumours of inducible colon carcinoma LS-shca9 cells with (+DOX) or without (–DOX) doxycycline in the drinking water of mice. Magnification 20×.
