Table 1.

PCR success in pooled and single-cell samples from two different experimental settings

	Female PBMNC of two samples		JAR		Interruption tissue		Interruption tissue
	Pooled cells (≤10)		Single cells		Pooled cells (5)		Single cells
	(n/total)	(%)	(n/total)	(%)	(n/total)	(%)	(n/total)	(%)
Successful PCR*	4/4	100	12/18	66.7	10/13	76.9	43/56	76.8
No. of heterozygous loci†	Varying		14		Varying		Varying
Amplification failure‡	1/64	1.6	52/192	27.1	10/160	6.3	190/688	27.6
Allele drop-in§ (all loci)	0/64	0	0/192	0	2/160	1.3	10/688	1.5
Allele drop-out** (heterozygous loci)	6/48	12.5	50/168	29.8	10/129	7.8	150/558	26.9
Heterozygous pattern†† (heterozygous loci)	41/48	85.4	78/168	46.4	109/129	84.5	252/558	45.2
PCR efficiency‡‡ (heterozygous loci)	88/96	91.7	206/336	61.3	228/258	88.4	654/1116	58.6

^*PCR yielding at least one amplification product.

^†Heterozygous loci of the respective samples as seen from PCR profiles (used for calculating ADO and PCR efficiency).

^‡Number and percentage of loci yielding no PCR fragment.

^§Extra peaks matching no profile. ^**Heterozygous loci yielding only one fragment (heterozygous loci only).

^††Loci showing two allele repeats (heterozygous loci only).

^‡‡Total number of PCR fragments calculated from heterozygous loci.