Table 2.

PCR efficiency based on the number of cells used as template

	Cell pools (≤10)		Cell pools (5)		Single cells		Overall
	(n/total)	(%)	(n/total)	(%)	(n/total)	(%)	(n/total)	(%)
Successful PCR*	4/4	100	10/13	76.9	55/74	74.3	69/91	75.8
Amplification failure†	1/64	1.6	10/160	6.3	242/880	27.5	253/1104	22.9
Allele drop-in‡ (all loci)	0/64	0	2/160	1.3	10/880	1.1	12/1104	1.1
Allele drop-out§ (heterozygous loci)	6/48	12.5	10/129	7.8	200/726	27.5	216/903	23.9
Heterozygous pattern** (heterozygous loci)	41/48	85.4	109/129	84.5	330/726	45.5	480/903	53.2
PCR efficiency†† (heterozygous loci)	88/96	91.7	228/258	88.4	860/1452	59.2	1176/1806	65.1

^**PCR yielding at least one amplification product.

^†Number and percentage of loci yielding no PCR fragment.

^‡Extra peaks matching no profile.

^§Heterozygous loci yielding only one fragment (heterozygous loci only).

^**Loci showing two allele repeats (heterozygous loci only).

^††Total number of PCR fragments calculated from heterozygous loci.