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. 2012 Dec 4;17(1):65–75. doi: 10.1111/j.1582-4934.2012.01646.x

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Fig. 6 — The spatial relationship between TCs and EMT-6 cells in EMT-6/stromal cells reconstituted breast cancer tissue was observed by double immunofluorescence staining. (A–C) c-kit/CD117⁺ cells was labelled with green colour and pan-CK⁺ cells was labelled with red colour. The nuclei were stained by Hoechst 33258. (D–F) Higher magnification of A, B and C. c-kit/CD117⁺ TCs distributed in the reconstituted breast cancer tissues (indicated by arrows), which are located close to pan-CK⁺ EMT-6 cells nest structure. EMT-6 cells were red stained and are indicated by arrowheads.