Fig. 9.

Apoptosis was detected by using TUNEL assay. Apoptosis index was measured by TUNEL assay in EMT-6 alone (A) and EMT-6/stromal cells reconstituted breast cancer tissue (B). Quantification of the apoptosis index of cells in EMT-6/stromal cells and EMT-6 alone reconstituted breast cancer tissues is shown in (C). At least three independent experiments were assessed. Bars show the standard error of the mean. Data were evaluated for significance through Student t tests; ANOVA was used to compare the data of cells going through apoptosis. Data were considered statistically significant at P < 0.05, which is indicated as *.