Table 1.

Real-time PCR primer sequences of a panel of genes involved in inflammatory response and housekeeping genes

Target genes	Primer sequences	GenBank accession #
Il1a	Forward 5′-GTGTTGCTGAAGGAGTTGCC-3′ Reverse 5′-CTGGATAAGCAGCTGATGTG-3′	NM_010554
Il1b	Forward 5′-GGACCCCAAAAGATGAAGGGCTGC-3′ Reverse 5′-GCTCTTGTTGATGTGCTGCTGCG-3′	NM_008361
Il-6	Forward 5′-CCTCTCTGCAAGAGACTTCC-3′ Reverse 5′-CTCCGGACTTGTGAAGTAGG-3′	NM_031168
Il-12b	Forward 5′-AACCAGAAAGGTGCGTTCCTC-3′ Reverse 5′-ATGCCCACTTGCTGCATGA-3′	NM_008352
TNF-α	Forward 5′-ATGGCCCAGACCCTCACACTCA-3′ Reverse 5′-TGGTGGTTTGCTACGACGTGGG-3′	NM_013693
Csf2	Forward 5′-TCGAGCAGGGTCTACGGGGC-3′ Reverse 5′-GTCCGTTTCCGGAGTTGGGGG-3′	NM_009969
Ccl2	Forward 5′-AGGTGTCCCAAAGAAGCTGTA-3′ Reverse5′-TCTGGACCCATTCCTTCTTG-3′	NM_011333
Cxcl1	Forward 5′-CCTGCAGACCATGGCTGGGAT-3′ Reverse 5′-GTGTGGCTATGACTTCGGTTTGGG-3′	NM_008176
Ccl3	Forward 5′-AGCTGACACCCCGACTGCCT-3′ Reverse 5′-TCAGGAAAATGACACCTGGCTGGGA-3′	NM_011337
Ccl4	Forward 5′-AGCCAGCTGTGGTATTCCTGACCA-3′ Reverse 5′-TCATGTACTCAGTGACCCAGGGCT-3′	NM_013652
Cxcl2	Forward 5′-GTTTGCCTTGACCCTGAAGCCCC-3′ Reverse 5′-CCAGGTCAGTTAGCCTTGCCTTTGT-3′	NM_009140
Gja1	Forward 5′-GGTCTGAGAGCCCGAACTCTCCT-3′ Reverse 5′-ACCCATGTCTGGGCACCTCTCTT-3′	NM_010288
Oxtr	Forward 5′-TCATCGTGTGCTGGACGCCT-3′ Reverse 5′-TGTTGAGGCTGGCCAAGAGCAT-3′	NM_001081147
Hpgd	Forward 5′-TCGGATTCACACGCTCAGCAGC-3′ Reverse 5′-TGTGTCCACAAAGCCTGGGCAA-3′	NM_008278
Ptgs2	Forward 5′-TGCCCAGCACTTCACCCATCA-3′ Reverse 5′-AGTCCACTCCATGGCCCAGTCC-3′	NM_011198.3
Il-10	Forward 5′-GCGGCTGAGGCGCTGTCAT-3′ Reverse 5′-GGCCTTGTAGACACCTTGGTCTTGG-3′	NM_010548
Hprt	Forward 5′-CAGTCCCAGCGTCGTGAT-3′ Reverse 5′-CAAGTCTTTCAGTCCTGTCCATAA-3′	NM_013556.2
Ppia	Forward 5′-CACCGTGTTCTTCGACATCA-3′ Reverse 5′-CCAGTGCTCAGAGCTCGAAAG-3′	NM_008907.1
Tbp	Forward 5′-TCCCAAGCGATTTGCTGCAGTCATC-3′ Reverse 5′-ACTCTTGGCTCCTGTGCACACCA-3′	NM_013684