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Indirect Immunofluorescence analysis of PARP-1 activity and AIF-dependent parthanatos. (A) ARPE-19 cells treated with either HMA or etoposide were probed with anti-PAR antibody to evaluate PAR synthesis. (B) Evaluation of AIF localization in HMA-treated ARPE-19 cells. Experiments were carried out with anti-mtHSP70 (to label mitochondria, red fluorescence) and anti-AIF (green fluorescence) antibodies. Scale bar 25 μm.
