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. 2009 Sep 14;14(10):2495–2505. doi: 10.1111/j.1582-4934.2009.00898.x

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© 2009 The Authors Journal compilation © 2010 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd

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Fig 6 — (A) Real-time PCR analysis of the marker genes of myogenesis, MyoD1, myogenin and Pax7, and myostatin at 3 and 7 days after injury. MyoD1, myogenin and Pax7 were significantly up-regulated in the miRNA group at 3 and 7 days. Myostatin was significantly down-regulated in the miRNA group at 3 and 7 days. Data were calculated as means ± S.D. *; P < 0.05. The P-value was determined by the Mann-Whitney U-test. (B) Immunofluorescent analysis of Pax7, desmin and MyoD1 in the axial section of both groups. An abundant number of Pax7⁺ cells around desmin⁺ myofibres were observed in the miRNA group. MyoD1⁺ cells were also abundant in the miRNA group compared to the control siRNA group. Bar: 50 μm.