(A) Real-time PCR analysis of the marker genes of myogenesis, MyoD1, myogenin and Pax7, and myostatin at 3 and 7 days after injury. MyoD1, myogenin and Pax7 were significantly up-regulated in the miRNA group at 3 and 7 days. Myostatin was significantly down-regulated in the miRNA group at 3 and 7 days. Data were calculated as means ± S.D. *; P < 0.05. The P-value was determined by the Mann-Whitney U-test. (B) Immunofluorescent analysis of Pax7, desmin and MyoD1 in the axial section of both groups. An abundant number of Pax7+ cells around desmin+ myofibres were observed in the miRNA group. MyoD1+ cells were also abundant in the miRNA group compared to the control siRNA group. Bar: 50 μm.