Fig 1.

Schematic structure and generation of K5 and its deletion mutants. (A) The primary sequence of K5 and its mutants. (B) The schematic structure of K5 and its mutants. Two deletion mutants were designed according to the structure and disulfide bond distribution of K5 (Pro452-Ala542). K5mut1 (Cys462-Cys541) retained the intact kringle domain and deleted the amino acid residues of both terminals outside kringle domain of K5. K5mut2 (Met463-Cys541) opened the first disulfide bond by deleting the amino acids of Cys462 on the basis of K5mut1 structure. (C) The production and identification of recombinant K5 and its mutant proteins. The top panel is SDS-PAGE with Coomassie blue staining while the bottom panel is Western blot analysis with antibody specific to His-tag; Marker: Protein marker; Lane1: Purified recombinant protein of K5; Lane2: Purified recombinant protein of K5mut1; Lane3: Purified recombinant protein of K5mut2.