Fig 3.

The gene expression changes and immuno-cytochemistry of p27 after c-MYC inhibition in hUCB-MSCs. (A) The protein expressions of CDK4 and PpRb are decreased after c-MYC inhibition. The expressions of p21 and p27 are increased after c-MYC inhibition. (B) The expressions of HDAC family genes in RT-PCR. (C) The expressions of HDAC2, HDAC4 and HDAC5 are decreased in real-time RT-PCR after c-MYC inhibition. (D) Western blot of HDAC2 after c-MYC inhibition. (E) Immunostaining of p27 after c-MYC knockdown. VC: vehicle control infected hUCB-MSCs; ShM3: c-MYC knocked-down hUCB-MSCs. Scale bar represents 50 μm.