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. 2011 Jun 22;15(7):1603–1614. doi: 10.1111/j.1582-4934.2010.01144.x

Fig 6.

Fig 6

ChIP assay in the HDAC2 promoter region and expression changes after the treatment of HDAC2 siRNA in c-MYC overexpressing hUCB-MSCs. (A) ChIP assay in hUCB-MSCs. The c-MYC binding site is present in the HDAC2 promoter region. (B) Western blot of HDAC2, p27, p57 and PpRb after HDAC2 siRNA treatment. The expression of HDAC2 is decreased after HDAC2 siRNA treatment. The expressions of p27 and p57 are up-regulated but the expression of PpRb is down-regulated after HDAC2 siRNA treatment in c-MYC overexpressing hUCB-MSCs compared with those of control-treated c-MYC overexpressing hUCB-MSCs. (C) The expressions of HDACs after HDAC2 siRNA treatment in c-MYC overexpressing hUCB-MSCs with RT-PCR. (D) Western blot of HDAC2 and HDAC4 in c-MYC knocked-down and overexpressed hUCB-MSCs before and after HDAC2 siRNA treatment. HDAC4 expression is decreased in both c-MYC knocked-down and overexpressed hUCB-MSCs without regarding to HDAC2 siRNA treatment. siHD2: HDAC2 siRNA treatment; VC: vehicle control lentivirus infected hUCB-MSCs; ShM3: c-MYC knocked-down hUCB-MSCs; OvM: c-MYC overexpressing hUCB-MSCs.