Fig 1.

Salermide triggered apoptosis in a concentration- and time-dependent manner. The indicated cell lines were seeded in 96-well microtitre plates and treated with the given concentration of salermide for 48 hrs. (A) Live cell number was estimated using SRB assay for calculation of cell survival. Points: mean of four replicate determinations; bars: S.D. (B) Cells were treated with indicated concentrations of salermide for 48 hrs (H157, H460 and Calu-1) or 24 hrs (H1650) and harvested for Western blot analysis. (C) H157 cells were treated with 50 μmol/l salermide for various lengths of time. Both attached and suspended cells were harvested for Western blot analysis; CF: cleaved form.