Fig 4.

Sirt1 and Sirt2 inhibition with salermide (A, B) or siRNA transfection (C) induces ER stress and DR5 up-regulation (C) in human NSCLC cells. H157 and Calu-1 cells were treated with 25/50 μmol/l salermide (H157) or 30/60 μmol/l salermide (Calu-1) for 48 hrs (A) or treated with 50 μmol/l salermide (H157) for the indicated hours (B). H157 cells were cultivated in a six-well plate and on the second day transfected with control, Sirt1, Sirt2 siRNA, respectively, or co-transfected with Sirt1 and Sirt2 siRNA. Cells were reseeded in a six-well plate on the third day (C) and treated with 50 μmol/L (H157) salermide for 48 hrs (C). Whole-cell protein lysates were harvested for Western blot analysis (A–C) and quantified using SensiAnsys software (A, B), *P < 0.05, **P < 0.01 as compared with control.