Fig 6.

Effects of salermide on the expression of ATF3- and DR5-dependent apoptosis in human NSCLC cell. The indicated cell lines were treated with the given concentrations of salermide for 48 hrs (H157, H460 and Calu-1) or 24 hrs (H1650). (B) H157 and Calu-1 cells were treated with 50 μmol/l (H157)/60 μmol/l (Calu-1) salermide for the given hours. Cells were subjected to preparation of whole-cell protein lysates. The given proteins were detected using Western blot analysis and evaluated by SensiAnsys. *P < 0.05, **P < 0.01 as compared with control (A, B). (C) H157 and Calu-1 cells were transfected as showed for 48 hrs, then cells were treated with 50 μmol/l (H157)/60 μmol/l (Calu-1) salermide for 48 hrs, the whole cells were harvested and molecular change about apoptosis were tested using Western blot. (D) The indicated cell lines were transfected with ATF3 siRNA and treated with the given concentrations of salermide. After 48 hrs, cell number was estimated using the SRB assay for calculation of cell survival. Points: mean of four replicate determinations; bars: S.D.