Fig 4.

The scheme for the synthesis of AG30/5C. (A) The AG30/5C molecule was assembled from four segments, that is (1–10), (11–17), (18–23) and (24–30), and each segment was elongated stepwise using the EDC/HOBt method as coupling reagents. (B) A large-scaled procedure employing the conventional solution method that is applicable for Good Manufacturing Practice toward clinical application in both synthetic and purification steps. The crude product was purified by ion-exchange chromatograpy followed by RP-HPLC. The purified product was passed successively through columns of Muromac and Sephadex G-10.