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. Author manuscript; available in PMC: 2014 Jul 30.
Published in final edited form as: Biochemistry. 2013 Jul 19;52(30):10.1021/bi4005182. doi: 10.1021/bi4005182

Figure 4.

Figure 4

The crystal structure of hGAR Tfase in both apo and 10R-, 10S-methylthio-DDACTHF liganded forms. A) Ribbon diagram of the overall topology of unliganded hGAR Tfase. Helices and β-sheets are shown as red coils and yellow strands, respectively. B) Superposition of apo hGAR Tfase (red) with hGAR Tfase in complex with 10S-methylthio-DDACTHF (green) and 10R-methylthio-DDACTHF (blue). C) Superposition of new high-resolution apo hGAR Tfase at 1.52 Å with previous apo GAR Tfase structure at 1.75 Å (1MEO)(7) showing the conformation of the previously disordered folate-binding loop 141-146. D) Close up view of the folate-binding loop 141-146 as determined in the apo hGAR Tfase structure at 1.52 Å. Figures generated using MOE 2011.10. (Chemical Computing Group, Canada).