Time courses of cytosolic pH (A) and Ca2+ concentration (B) in EC exposed to simulated ischaemia in bicarbonate- buffered medium without (Bic, O-) or with DIDS (Bic + DIDS, 300 μmol/l, -Δ-), or in HEPES-buffered medium (Hep, -•-). Values are mean ± SEM, n = 5–6. *–P < 0.05 versus Hep and Bic + DIDS.