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Time course of mitochondrial membrane potential measured with fluorescent dye JC-1 in EC exposed to simulated ischaemia in bicarbonate-buffered medium without (Bic, -O-) or with DIDS (Bic + DIDS, 300 μmol/l, -•-), or in HEPES-buffered medium (Hep, -•-). Values are mean ± SEM, n = 4–5. *–P< 0.05 versus Hep and Bic + DIDS. #–P<0.05 versus Bic + DIDS.