1.

FACS analysis of integrin beta1 subtype expression on Caki-I, KTC-26 and A498 tumour cells. Cells were washed in blocking solution and then stained with specific monoclonal antibodies as listed in Materials and methods. A mouse IgG1-FITC was used as an isotype control for FITC conjugated antibodies. To evaluate background staining of PE conjugated antibodies, goat, anti-mouse IgG1-PE was used. Fluorescence was analyzed using a FACScan flow cytometer, and a histogram plot was generated to show FITC/PE-fluorescence. One from 6 independent experiments.