2.

Down-regulation of alpha integrins on RCC cell lines co-cultivated with HUVEC. Cell cultures were double stained using the HUVEC specific Factor VIII-associated antigen monoclonal antibody (clone F8/86) and monoclonal antibodies directed against alpha2, alpha3 or alpha5 integrins. Both cell populations were defined in the FL-1 versus FL-2 dot blot diagram (Fig. 2A, representative for A498) and tumour cells (VIII-) analyzed separately. Dynamics of integrin expression is depicted in Fig. 2B as MFU values.