4.

Endothelial membrane fragments but not cell culture supernatant reduce alpha3 and alpha5 surface expression and alpha2, alpha3 and alpha5 mRNA of A498 tumour cells. Surface level was analyzed by flow-cytometry and expressed as MFU values.*indicates significant difference to controls (n = 6). RNA was extracted, reverse-transcribed and submitted to semi-quantitative reverse transcription-PCR using gene-specific primers as described in Materials and methods. The internal control for the RT-PCR reaction was performed by running parallel reaction mixtures with the housekeeping gene GAPDH. One representative of three separate experiments is shown.